Figure 3. Two-week-old embryoid bodies (EBs) exhibit defects in the expression of endoderm markers.
Tissue sections of 2-week-old EBs were immunostained using primary antibody to the indicated markers. Expression of the ectoderm markers, Nestin (a, d), Keratin 18 (K18) (b, e) and β-Tubulin III (e, f); mesoderm marker, Desmin (g, j) and endoderm markers, α-Fetoprotein (AFP) (h, k) and ApoE (i, l) are indicated. Scale bars: 20 µm