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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Dev Biol. 2013 Dec 28;386(2):448–460. doi: 10.1016/j.ydbio.2013.12.038

Figure 4. Hsbp1−/− ES cell differentiation into neural tissue in vitro.

Figure 4

(A) Phase-contrast photographs of differentiated hsbp1+/+ and hsbp1−/− ES cells (a–b). Scale bar: 50 µm.

(B) Immunofluorescence staining for β-Tubulin III (Red; c–d) and Neu N (Green; e–f), and nuclear staining (DAPI, blue) of differentiated hsbp1+/+ and hsbp1−/− ES cells. Merged immunofluorescence of β-Tubulin III and NeuN are presented in g–h. Scale bars: 50 µm.

(C) Immunofluorescence staining of Map2 in differentiated hsbp1+/+ (i) and hsbp1−/− (j) ES cells. Scale bars: 20 µm

(D) Quantification of NeuN, β-Tubulin III and Map2 in differentiated hsbp1+/+ and hsbp1−/− ES cells. Bars are mean +/− SEM. Statistical analyses were performed using Student t-tests. ***p<0.0001.