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. Author manuscript; available in PMC: 2014 Jul 29.
Published in final edited form as: Anal Biochem. 2006 Nov 27;361(2):218–225. doi: 10.1016/j.ab.2006.11.011

Fig. 4.

Fig. 4

Fluorophore-assisted carbohydrate electrophoresis. Lane 1 contains 3 nmol of HA4-ANDA and 30 nmol of both UDP-GlcNAc and UDP-GlcUA. Lane 2 contains 3 nmol of HA4-ANDA. Lane 3 contains 3 nmol of HA4-ANDA and 30 nmol of both UDP-GlcNAc and UDP-GlcUA in the presence of non-induced cell lysate. Lane 4 shows the extension of HA4-ANDA on the addition of 3 nmol of HA4-ANDA combined with 30 nmol of both UDP-GlcNAc and UDP-GlcUA in the presence of induced cell lysate. Lane 5 is a repeat of lane 4 except that the elongated HA4-ANDA fragments were not treated with acid or boiled prior to loading on a gel (see Materials and methods). The elongated HA4-ANDA fragment shown in lane 4 was digested with either Streptomyces (lane 6) or bovine testicular HAase (lane 7).