Skip to main content
. Author manuscript; available in PMC: 2014 Jul 29.
Published in final edited form as: Anal Biochem. 2006 Nov 27;361(2):218–225. doi: 10.1016/j.ab.2006.11.011

Table 1.

Apparent Michaelis–Menten constants

Substrate KM, app (mM)
UDP-G1cUA 0.014
UDP-G1cNAc 0.66
HA4 0.91

Note. Values in the table are averages of two measurements. Values were obtained using the standard assay protocol. KM, app values were obtained by varying the concentration of one substrate while keeping the other two substrates constant at near-saturating concentrations of ≥4KM, app. The reported KM, app values have errors less than 20%.