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. 1978 Mar;75(3):1153–1156. doi: 10.1073/pnas.75.3.1153

Multiple receptors for brain dopamine.

M Titeler, P Weinreich, D Sinclair, P Seeman
PMCID: PMC411427  PMID: 418405

Abstract

This study was done to obtain direct in vitro evidence for the possible existence of more than one type of dopaminergic binding site in homogenates of the caudate nucleus from calf brain. Five radioligands for dopaminergic sites were tested. The inhibitions of two agonist radioligands ([3H]dopamine, [3H]apomorphine) by haloperidol, chlorpromazine, or piflutixol were biphasic. The inhibitions of [3H]haloperidol, [3H]spiroperidol, and [3H]dihydroergocryptine binding by dopamine and (-)-norepinephrine were also biphasic. Thus, both 3H-labeled agonists and 3H-labeled antagonists were possibly binding to two high-affinity sites.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bunney B. S., Aghajanian G. K., Roth R. H. Comparison of effects of L-dopa, amphetamine and apomorphine on firing rate of rat dopaminergic neurones. Nat New Biol. 1973 Sep 26;245(143):123–125. doi: 10.1038/newbio245123a0. [DOI] [PubMed] [Google Scholar]
  2. Burt D. R., Creese I., Snyder S. H. Properties of [3H]haloperidol and [3H]dopamine binding associated with dopamine receptors in calf brain membranes. Mol Pharmacol. 1976 Sep;12(5):800–812. [PubMed] [Google Scholar]
  3. Burt D. R., Enna S. J., Creese I., Snyder S. H. Dopamine receptor binding in the corpus striatum of mammalian brain. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4655–4659. doi: 10.1073/pnas.72.11.4655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carlsson A., Kehr W., Lindqvist M., Magnusson T., Atack C. V. Regulation of monoamine metabolism in the central nervous system. Pharmacol Rev. 1972 Jun;24(2):371–384. [PubMed] [Google Scholar]
  5. Cools A. R., Boudier A. J., Rossum J. M. Dopamine receptors: selective agonists and antagonists of functionally distinct types within the feline brain. Eur J Pharmacol. 1976 Jun;37(2):283–293. doi: 10.1016/0014-2999(76)90036-4. [DOI] [PubMed] [Google Scholar]
  6. Fields J. Z., Reisine T. D., Yamamura H. I. Biochemical demonstration of dopaminergic receptors in rat and human brain using [3H]spiroperidol. Brain Res. 1977 Nov 18;136(3):578–584. doi: 10.1016/0006-8993(77)90084-1. [DOI] [PubMed] [Google Scholar]
  7. Kehr W., Carlsson A., Lindqvist M. Catecholamine synthesis in rat brain after axotomy: interaction between apomorphine and haloperidol. Naunyn Schmiedebergs Arch Pharmacol. 1977 Mar;297(2):111–117. doi: 10.1007/BF00499920. [DOI] [PubMed] [Google Scholar]
  8. Laduron P., Leysen J. Specific in vivo binding of neuroleptic drugs in rat brain. Biochem Pharmacol. 1977 May 15;26(10):1003–1007. doi: 10.1016/0006-2952(77)90486-5. [DOI] [PubMed] [Google Scholar]
  9. Seeman P. Anti-schizophrenic drugs--membrane receptor sites of action. Biochem Pharmacol. 1977 Oct 1;26(19):1741–1748. doi: 10.1016/0006-2952(77)90340-9. [DOI] [PubMed] [Google Scholar]
  10. Seeman P., Chau-Wong M., Tedesco J., Wong K. Brain receptors for antipsychotic drugs and dopamine: direct binding assays. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4376–4380. doi: 10.1073/pnas.72.11.4376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Seeman P., Chau-Wong M., Tedesco J., Wong K. Dopamine receptors in human and calf brains, using [3H]apomorphine and an antipsychotic drug. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4354–4358. doi: 10.1073/pnas.73.12.4354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Seeman P., Lee T., Chau-Wong M., Wong K. Antipsychotic drug doses and neuroleptic/dopamine receptors. Nature. 1976 Jun 24;261(5562):717–719. doi: 10.1038/261717a0. [DOI] [PubMed] [Google Scholar]
  13. Snyder S. H., Creese I., Burt D. R. The brain's dopamine receptor: labeling with (3H) dopamine and (3H) haloperidol. Psychopharmacol Commun. 1975;1(6):663–673. [PubMed] [Google Scholar]
  14. Snyder S. H. The dopamine hypothesis of schizophrenia: focus on the dopamine receptor. Am J Psychiatry. 1976 Feb;133(2):197–202. doi: 10.1176/ajp.133.2.197. [DOI] [PubMed] [Google Scholar]
  15. Tittler M., Weinreich P., Seeman P. New detection of brain dopamine receptors with (3H)dihydroergocryptine. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3750–3753. doi: 10.1073/pnas.74.9.3750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Walters J. R., Roth R. H. Dopaminergic neurons: an in vivo system for measuring drug interactions with presynaptic receptors. Naunyn Schmiedebergs Arch Pharmacol. 1976 Dec;296(1):5–14. doi: 10.1007/BF00498834. [DOI] [PubMed] [Google Scholar]

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