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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Neuromuscul Disord. 2014 May 22;24(8):693–706. doi: 10.1016/j.nmd.2014.05.006

Figure 6. Lemaire D region is necessary for high expression in C2C12 myotubes.

Figure 6

(A) Diagram of the pHRL 3’UTR constructs with portions of the Lemaire D region deleted (dl-D, dl-Da, dl-Db, dl-Dc). Lemaire D and the vigilin binding site overlap the Db and Dc regions. A construct containing six point mutations in the vigilin binding site was made (Vm). (B) Relative expression levels from day six C2C12 myotubes transfected with the pHRL Renilla construct (pHRL), the full-length 3’UTR construct (3’UTR), or Lemaire D mutant constructs (dl-Da, dl-Db, dl-Dc, and Vm containing 6 point mutations in the vigilin binding site) is shown. Relative expression levels for each construct were measured using a dual-luciferase assay as previously described. The average of four biological transfection replicates is shown for each construct. Error bars equal +/- 1 standard deviation.