Skip to main content
. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Neuromuscul Disord. 2014 May 22;24(8):693–706. doi: 10.1016/j.nmd.2014.05.006

Figure 8. The DMD 5’UTRs sequences cause suboptimal translation initiation.

Figure 8

(A) Diagram of pHRL Renilla vectors containing the DMD 3’UTR and the dp427m muscle 5’UTR (5’UTRM), the dp427p brain 5’UTR (5’UTRP), or the dp427c brain 5’UTR (5’UTRC) inserted immediately upstream of the hRluc Renilla coding sequence in the pHRL-CMV vector. (B) Relative expression levels for C2C12 myoblasts or myotubes transfected with either the control Renilla reporter construct (pHRL) or a Renilla reporter construct containing the dp427m 5’UTR and the full-length DMD 3’UTR (5’UTRM) are shown. (C) Relative expression levels in C2C12 myoblasts or myotubes transfected with a reporter construct containing either the dp427m muscle 5’UTR (5’UTRM) or the 5’UTRs of the full-length dp427p and dp427c brain isoforms (5’UTRP, 5’UTRC) are shown. The average of four biological transfection replicates is shown for each construct at each time point. Error bars equal +/- 1 standard deviation.