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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Immunol. 2014 Jun 18;193(2):688–699. doi: 10.4049/jimmunol.1302059

Figure 2. Eomesodermin+ CD4SP thymocytes produce IFNγ in response to stimulation.

Figure 2

Thymocytes from WT and itk-/- mice were stimulated with 10 ng/mL PMA and 1 μg/mL ionomycin for six hours, and then stained with CD1d-tetramer and antibodies against CD4, CD8, HSA, IFNγ, and Eomesodermin. Dot-plots are gated on CD4SP CD1d-tetramerneg HSAlow thymocytes, and show IFNγ versus Eomes staining. Results are representative of two independent experiments involving 2-4 mice per group.