Figure 3. CD4⁺ Eomes⁺ T cells are MHC class II-dependent.

Thymocytes from WT, itk^-/-, H2^dlAb1-Ea, and itk^-/-/H2^dlAb1-Ea mice were stained with CD1d-tetramer and antibodies to CD4, CD8, TCRδ, TCRβ, HSA, CD44, and Eomesodermin. Dot-plots show Eomes versus CD44 staining, and graphs show compilations of the percentages and absolute numbers of Eomes⁺ cells.

(A) Total thymic cellularity. Significant differences were seen between H2^dlAb1-Ea and itk^-/-/H2^dlAb1-Ea mice (p < 0.005).

(B) Gated on CD8SP TCRβ^high HSA^low thymocytes.

(C) Gated on CD4SP TCRδ^neg TCRβ^high HSA^low CD1d-tetramer^neg thymocytes. n = 6-8 mice per group. Results are representative of three independent experiments. Statistical analysis was performed using a one-way ANOVA. **p < 0.005 ***p < 0.0005 ****p < 0.00001