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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Immunol. 2014 Jun 18;193(2):688–699. doi: 10.4049/jimmunol.1302059

Figure 4. αβ NKT cells promote the development of Eomesodermin+ T cells.

Figure 4

Thymocytes from WT, itk-/-, cd1d-/-, and itk-/-cd1d-/- mice were stained with CD1d-tetramer and antibodies against CD4, CD8, TCRδ, TCRβ, HSA, CD44, and Eomesodermin. Dot-plots show Eomes versus CD44 staining, and graphs show compilations of the percentages and absolute numbers of Eomes+ cells.

(A) Total thymic cellularity. Significant differences were seen between WT and CD1d KO mice (p < 0.05).

(B) Gated on CD8SP TCRβhigh HSAlow thymocytes.

(C) Gated on CD4SP TCRδneg TCRβhigh HSAlow CD1d-tetramerneg thymocytes. n = 7-9 mice per group. Results are from four independent experiments. Statistical analysis performed using a one-way ANOVA. *p < 0.05 ***p < 0.0005 ****p < 0.0001