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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Immunol. 2014 Jun 18;193(2):688–699. doi: 10.4049/jimmunol.1302059

Figure 5. Eomesodermin+ T cells develop independently of γδ T cells.

Figure 5

Thymocytes from WT, itk-/-, tcrd-/-, and itk-/-tcrd-/- mice were stained with CD1d-tetramer and antibodies to CD4, CD8, TCRβ, HSA, CD44, and Eomesodermin. Dot-plots show Eomes versus CD44 staining, and graphs show compilations of the percentages and absolute numbers of Eomes+ cells.

(A) Total thymic cellularity. No significant differences were detected.

(B) Gated on CD8SP TCRβhigh HSAlow thymocytes.

(C) Gated on CD4SP TCRβhigh HSAlow CD1d-tetramerneg thymocytes.

n = 4-7 mice per group. Results are representative of two independent experiments. Statistical analysis was performed using a one-way ANOVA. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001