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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Immunol. 2014 Jun 18;193(2):688–699. doi: 10.4049/jimmunol.1302059

Figure 6. CD4+ and CD8+ Eomes+ T cells are dependent on IL-4.

Figure 6

Thymocytes from WT, itk-/-, il4ra-/-, and itk-/-il4ra-/- mice were stained with antibodies to CD4, CD8, TCRβ, HSA, CD44, and Eomesodermin. Dot-plots show Eomes versus CD44 staining, and graphs show compilations of the percentages and absolute numbers of Eomes+ cells.

(A) Total thymic cellularity. Significant differences were detected between WT and itk-/- (p < 0.0001), WT and itk-/-il4ra-/- (p < 0.0005), itk-/- and il4ra-/-(p < 0.0005), and il4ra-/- and itk-/-il4ra-/- (p < 0.005).

(B) Gated on CD8SP TCRβhigh CD24(HSA)low thymocytes.

(C) Gated on CD4SP TCRβhigh CD24(HSA)low thymocytes.

n = 3-6 mice per group. Results are representative of three independent experiments. Statistical analysis was performed using one-way ANOVA. ***p < 0.0001