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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Immunol. 2014 Jun 18;193(2):688–699. doi: 10.4049/jimmunol.1302059

Figure 8. Development of all innate T cells in itk-/- is dependent on the SLAM-family receptor adapter protein, SAP.

Figure 8

Thymocytes from WT, itk-/-, sh2d1a-/-, and itk-/- sh2d1a-/- mice were stained with CD1d-tetramer and antibodies against CD4, CD8, TCRβ, HSA, Eomesodermin, and PLZF.

(A) Total thymic cellularity. Significant differences were seen between WT and itk-/- mice (p < 0.005), WT and sh2d1a-/- mice (p < 0.05), and WT and itk-/- sh2d1a-/- mice (p < 0.0005).

(B) Dot-plots show PLZF versus Eomes staining on CD4SP TCRβhigh HSAlow CD1d-tetramerneg thymocytes.

(C-D) Graphs show frequencies (C) and absolute numbers (D) of CD4 SP TCRβhigh HSAlow CD1d-tetramerneg cells expressing PLZF (left) or Eomes (right). n = 10-12 mice per group. Results are representative of four independent experiments. Statistical analysis was performed using a one-way ANOVA. ****p < 0.0001