Figure 4. Normal Pk localization in bbs7 knockown embryos and normal BBsome formation in Pk2−/− mouse tissues.
A–B. GFP-Pk localization in the developing neural tube at 24hpf. A, GFP-Pk localization. B, GFP-Pk localization with bbs7 MO injected. Asterisks mark cells with anterior GFP-Pk puncta. Anterior of the embryo is oriented to the top. Scale bars are 30μm. C. Quantification of percentage of GFP-positive cells with anterior puncta. For control, n=53; bbs7 MO, n=41. D. Immunoprecipitation analysis demonstrates BBSome formation in Pk2 mutants.