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. Author manuscript; available in PMC: 2014 Jul 29.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Dec 9;31(3):616–623. doi: 10.1161/ATVBAHA.110.212621

Figure 1.

Figure 1

Effects of PDE1 inhibition on collagen I protein levels in human SV explants and VSMCs cultured on polymerized or monomeric collagen matrix. A, Images of human SVs. Sections of human SVs, before or after culture for 7 days in the presence of vehicle or 30 μmol/L IC86340, were subjected to Verhoeff Masson Trichrome combination staining. Int indicates intima; Med: media; Adv: adventitia. B, Western blotting showing IC86340 decreased collagen I expression in cultured human SV. Collagen I protein levels were detected by Western blotting using collagen I–specific antibody (kindly provided by Dr Fisher, National Institutes of Health). Band intensities were quantified, and results are shown as mean±SD (n=6). C, Collagen I protein levels in VSMCs cultured on polymerized collagen matrix or on monomeric collagen matrix. D, The effect of IC86340 on collagen I expression. After being cultured on polymerized or monomeric collagen with vehicle or 15 μmol/L IC86340 for 48 hours, cells were harvested, and the expression of collagen I and smooth muscle calponin was examined by Western blotting.