Figure 1.

A significant proportion of tumor‐infiltrating CD4⁺ T Cells are CD62L^hiCCR7⁺CD44^lo. Tumor‐cell suspensions were stained with CD4‐, Foxp3‐, CD62L‐ and CCR7‐specific monoclonal antibodies (mAbs) and analyzed by flow cytometry. Proportion of CD4⁺ cells that are Foxp3⁻ or Foxp3⁺ are shown (a). Examples of CD62L and CCR7 staining on gated CD4⁺Foxp3⁺ and CD4⁺Foxp3⁻ cells (b and c). Proportions of tumor‐infitrating CD4⁺Foxp3⁺and Foxp3⁻ cells from spleens, tumors, draining and nondraining lymph nodes, represented as CD62L^hiCCR7⁺, CD62L^loCCR7⁺ and CD62L^loCCR7⁻ (d and e) (n = 7). Expression of CD44 by CD62L^hi and CD62L^lo cells (f).