Figure 6.

The proportion of intratumoral naïve T cells increases significantly with tumor progression. Tumor cell suspensions were stained with CD4‐, CD44‐, CCR7‐ and CD62L‐specific mAbs and analyzed by flow cytometry. Proportions of CD4⁺CD44^loCD62L⁺ cells were analyzed with respect to tumor weight at the time of sample preparation (a). Frequency of naïve CD4⁺ TILs in small versus large tumor (b). In (c), Foxp3⁺ cells were excluded from the analysis to more accurately demonstrate the relationship between naïve CD4⁺ cells and tumor weight. The relationship between CD4⁺Foxp3⁺ cells and tumor weight (d). The relationship between activated effector CD4⁺Foxp3⁻ TILs and tumor weight (e).