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. 2014 Jul 29;9(7):e103245. doi: 10.1371/journal.pone.0103245

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© 2014 Mills et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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a. The relationship between tumor rotation angle, , and projected aspect ratio, , as an oblate ellipsoid with maximum aspect ratio of 3 ( = 3) is rotated about its () axis. b. The tumor rotation angle was calculated from the projected aspect ratio (part a) for tumors grown in 1% agarose for 30 days. Top image: observation perpendicular to the plane of the cell-culture well, Bottom image: side view perpendicular to a physical cross-section of the gel made with a scalpel blade (out-of-focus cut marks are visible in the gel) Scale bars are 200 µm.

Inline graphic — a. The relationship between tumor rotation angle, , and projected aspect ratio, , as an oblate ellipsoid with maximum aspect ratio of 3 ( = 3) is rotated about its () axis. b. The tumor rotation angle was calculated from the projected aspect ratio (part a) for tumors grown in 1% agarose for 30 days. Top image: observation perpendicular to the plane of the cell-culture well, Bottom image: side view perpendicular to a physical cross-section of the gel made with a scalpel blade (out-of-focus cut marks are visible in the gel) Scale bars are 200 µm.