Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 21;69(6):730–740. doi: 10.1111/all.12392

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Authors. Allergy published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

Phenotyping and retroviral gene transduction of mesenchymal stem cells (MSCs). (A) Cell surface marker determination by flow cytometry. X‐axes represent fluorescence intensity, and black histograms are from isotype‐matched controls. Following harvesting and culture in MSC selective medium, the adherent cell population showed a Sca‐1⁺CD44⁺CD106⁺CD11b⁻CD45⁻CD14⁻ cell surface marker phenotype. (B) The cells produced calcified extracellular matrix upon culture in osteogenic differentiation medium as demonstrated by alizarin‐red staining (upper‐right image) or fat deposits upon culture in adipogenic differentiation medium as shown by oil‐red O staining (lower‐right image). Upper‐left and lower‐left micrographs show the respective stainings on cells cultured in control media. (C) The MSCs were efficiently transduced to express green fluorescent protein employing a murine stem cell virus retroviral vector. Data are representative of three independent experiments.