Figure 8. HO-1 modulates CO-induced STAT3 glutathionylation.
A. ECs were transiently transfected with pcDNA vector or HO-1 plasmid. S-glutathionylated STAT3 was immunoprecipitated and detected using a protein-SSG antibody. *P<0.05 compared with untreated ECs. B. ECs were transfected with control or HO-1 siRNA vectors for 36 hours and then exposed to TCDC or MC for 12 hours. S-glutathionylated STAT3 was then detected as above. *P<0.05 compared with untreated ECs. # P<0.05 compared with TCDC or MC alone.