Figure 1.

Domain structure of ColN.

A. Schematic diagram of ColN highlighting T‐domain (blue), R‐domain (green) and P‐domain (amber) and their abbreviated construct names in ColN.

B. Amino acid sequence of ColN‐R (91–184 aa) highlighting positively charged amino acids in red.

C. PDB structures of (a) ColN (1A87) colouring as above and missing the disordered 90 residues of the T‐domain. Showing the residues mutated to cysteines to form the disulphide bridge (V94 and Q179) as space filling. Also at the same scale a representative LPS structure equivalent to Rd plus Hep(III) (see Fig. 4) from the structure of the TLR4‐MD‐2‐E. coli LPS complex 3FXI (Park et al., 2009).