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. 2014 Jul 29;9(7):e96441. doi: 10.1371/journal.pone.0096441

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© 2014 Xu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: Strong cytoplasmic staining for Shh in pancreatic cancer cells (×400); B: Weakly positive staining for Shh in ductal complex of chronic pancreatitis tissues (×400); C: Weakly positive staining for Shh in islet cells of normal cancer side tissue (×200); D: Negtive staining for Shh in normal ductal epithelial/acinar cells of cancer side tissue (×200); E: Cancer cells show strong nuclear staining for Gli1 and normal cancer side tissues were negtive (×400); F: Strong positive perinuclear cytoplasmic and part nuclear staining for Gli1 in pancreatic cancer cells (×400); G: Strong positive cytoplasmic staining for S100A4 in pancreatic cancer cells (×400); H: Positive cytoplasmic staining for E-cadherin in pancreatic cancer cells. Black arrows point to positive staining area and white arrows point to negtive staining area.