Table 1. The primer sequences for real time RT-PCR assays.

Gene	primer sequences
Gli1	F: 5'-TCTGCCCCCATTGCCCACTTG-3'
	R: 5'-TACATAGCCCCCAGCCCATACCTC-3'
Shh	F: 5'-CGGAGCGAGGAAGGGAAAG-3'
	R: 5'-TTGGGGATAAACTGCTTGTAGGC-3'
Patched1	5'-CGGCGTTCTCAATGGGCTGGTTTT-3'
	5'-GTGGGGCTGCTGTTTCGGGTTCG-3'
GAPDH	F: 5'-ACGGATTTGGTCGTATTGGG-3'
	R: 5'-TGGAAGATGGTGATGGGATT-3'
E-cadherin	F: 5'- CAATGCCGCCATCGCTTAC -3'
	R: 5'- CAAAATGCCATCGTTGTTCACT -3'
Vimentin	F: 5'- CAATGAGTCCCTGGAACGCC -3'
	R: 5'- CACGAAGGTGACGAGCCATT -3'
S100A2	F: 5'- CGGTCCAGGATGCCCAGTC -3'
	R: 5'- GGCTCCCAGGGTGAGGATTTAT -3'
S100A4	F: 5'- ACTCGGGCAAAGAGGGTGA -3'
	R: 5'- CTGGGCTGCTTATCTGGGAA -3'
S100A6	F: 5'- GGGGAGACTCGTCACCAGGC -3'
	R: 5'- GGTCCAAGTCTTCCATCAGCCT -3'
S100A10	F: 5'- GCCGCCTCCCTCTACCCAC -3'
	R: 5'- GCCTTTATCCCCAGCGAATTT -3'
S100A11	F: 5'- AGGGCGTGGGTTGAGGAGA -3'
	R: 5'- TGTGGAGATGATGACAGAAAGGC -3'
S100A13	F: 5'- GCTGGGAGGAGCGGTTAGA -3'
	R: 5'- GCAAAGGTGAAGAAGGTGGTGA -3'
S100A14	F: 5'- CAGTGTCGGTCAGCCAACG -3'
	R: 5'- TCCCAGAAACTCCTGAACTCCA -3'
S100A16	F: 5'- TGAACTGGGGTCCCTTTGTG -3'
	R: 5'- TCAGGACGGACCCAGAATCA -3'
S100P	F: 5'- AAGAGGCTGCCAGTGGGACA -3'
	R: 5'- TTGGTCAGGGTCTGCGTGC -3'
TCHH	F: 5'- TTCGGAGACCACATGACCCTA -3'
	R: 5'- GCTCTTCCCGTTCTTGCCA-3'