Figure 2. Effect of capsaicin on TRP expression and Ca²⁺ influx in 3T3-L1 cells.

(A) Normalized mRNA expression levels of TRPV1, TRPV4, TRPC1 and TRPC5 in 3T3-L1 cells differentiated for 8 days; (B) Change in TRPV1 expression levels in adipocyte when differentiated in presence of capsaicin. (C & D) Effect of TRPV1 agonist, Cap and RTX, and antagonist, Cpz, on Ca²⁺ influx in pre-adipocytes. All values are expressed as mean ± S.E.M. (n = 3). *P<0.05 as compared to control or pre-adipocyte (wherever mentioned in panel), ^†P<0.05 as compared to adipocyte. TRPV =  transient receptor potential channel vanaloid type, TRPC =  transient receptor potential channel canonical type, Cap =  Capsaicin, RTX =  resiniferatoxin, Cpz =  Capsazepine.