TABLE 1.
Sequences of mutagenesis oligonucleotides
| Oligos | Sequences (5′ to 3′) |
|---|---|
| IR1-sensea,b | GTCTTGGGCTGCCCTTAGACGCGTGAATTCTTAGGCAAATAGATAAT |
| IR1-antisense | ATTATCTATTTGCCTAAGAATTCACGCGTCTAAGGGCAGCCCAAGAC |
| R331L-sense | TGCGGTTGAAGCTATGTTCCTTCTTTCAGCTGAGATTTTCAATAAGAAAC |
| R331L-antisense | GTTTCTTATTGAAAATCTCAGCTGAAAGAAGGAACATAGCTTCAACCGCA |
| L340Y-sense | CAGCTGAGATTTTCAATAAGAAATATCCGTCTGGGCATTCTGACCTATTGG |
| L340Y-antisense | CCAATAGGTCAGAATGCCCAGACGGATATTTCTTATTGAAAATCTCAGCTG |
| R351L-sense | GGGCATTCTGACCTATTGGAAGAACTAATTCGAAATAGTGGTATCTCTGAT |
| R351L-antisense | ATCAGAGATACCACTATTTCGAATTAGTTCTTCCAATAGGTCAGAATGCCC |
| I352Y-sense | CATTCTGACCTATTGGAAGAAAGATATCGAAATAGTGGTATCTCTGATGA |
| I352Y-antisense | TCATCAGAGATACCACTATTTCGATATCTTTCTTCCAATAGGTCAGAATG |
a
The IR1 element in the BSEP promoter is underlined.
b
The mutated nucleotides are in bold.