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. 2014 Jun 19;8(3):1269–1274. doi: 10.3892/ol.2014.2270

Figure 3.

Figure 3

A549/CDDP cells were pre-incubated with 10 μM SB203580 for 2 h, followed by incubation with 4 mM Met for 24 h. (A) Cells were stained with Hoechst 33258 (x200). (B) The apoptotic index was determined by calculating the percentage of the apoptotic nuclei as a proportion of the total nuclei. Data are presented as the mean ± standard deviation (n=4). (C) The expression levels of proteins as determined by western blotting. Met, metformin; MAPK, mitogen-activated protein kinase; PARP, poly (ADP-ribose) polymerase; Bid, BH3 interacting-domain death agonist; SB, SB203580 p38 MAPK inhibitor.