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. 2014 Jun 30;8(3):1163–1168. doi: 10.3892/ol.2014.2300

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Copyright © 2014, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Enforced overexpression of Nek6 promotes the proliferation of HCC cells. (A) The protein level of Nek6 in Huh7 and PLC/PRF/5 cells with pcDNA3.1B-Nek6 transfection. Overexpression of Nek6 (B) enhanced the cell viability and (C) promoted the colony formation of Huh7 and PLC/PRF/5 cells, respectively. (D) Quantification of the colony formation. Data are presented as the mean ± standard deviation of three independent experiments. ^**P<0.01, vs. the control. Nek6, never in mitosis gene A-related kinase 6; HCC, hepatocellular carcinoma; OD, optical density.