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. 2014 Jun 30;8(3):1163–1168. doi: 10.3892/ol.2014.2300

Figure 3.

Figure 3

Knockdown of Nek6 inhibits the proliferation of hepatocellular carcinoma cells. (A) The protein level of Nek6 in Hep3B and HepG2 cells with siRNA transfection were evaluated by western blot analysis. The expression of endogenous Nek6 was markedly reduced by Nek6 knockdown. (B) Knockdown of Nek6 inhibited the cell viability of Hep3B and HepG2 cells. (C) Gene silencing of Nek6 weakened the colony formation of Hep3B and HepG2 cells. (D) Quantification of the colony formation. Data are presented as the mean ± standard deviation of three independent experiments. **P<0.01, vs. the control. Nek6, never in mitosis gene A-related kinase 6; NC, negative control; OD, optical density; siRNA, small interfering RNA; shRNA, short hairpin RNA.