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PCR primers used in reaction.
| Target | Primer | Sequence (5′-3′) | Length of amplicon (bp) | Tm (°C) |
|---|---|---|---|---|
| TβRI | Forward | GCAGTAAGACATGATTCAGCCACAG | 190 | 58.1 |
| Reverse | CAATGGAACATCGTCGAGCAA | |||
| TβRII | Forward | GAAATTCCCAGCTTCTGGCTCA | 143 | 57.2 |
| Reverse | CTGTCCAGATGCTCCAGCTCAC | |||
| Smad3 | ||||
| Forward | CCAGGGCTTTGAGGCTGTCTA | 143 | 59.2 | |
| Reverse | GCAAAGGCCCATTCAGGTG | |||
| β-actin | Forward | TGGCACCCAGCACAATGAA | 186 | 56.0 |
| Reverse | CTAAGTCATAGTCCGCCTAGAAGCA | |||
