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. 2014 Jun 17;8(3):1217–1221. doi: 10.3892/ol.2014.2259

Figure 1.

Figure 1

Expression of HIF-1α and its downstream genes, VEGF, EPO and CXCR4. (A) Western blot analysis was used to detect the effect of dn HIF-1α and fL HIF-1α on VEGF, EPO and CXCR4 expression. β-actin was used as a loading control. VEGF, EPO and CXCR4 were upregulated by fL HIF-1α, while dn HIF-1α significantly downregulated the HIF-1 target genes. (B) The relative protein expression of HIF-1α, VEGF, EPO and CXCR4 was calculated as integrated density values. Data are presented as the mean ± standard error of the mean from three independent experiments. *P<0.05 vs. pcDNA3.1 or wild type. HIF, hypoxia-inducible factor; VEGF, vascular endothelial growth factor; EPO, erythropoietin; CXCR4, CXC chemokine receptor 4; fL, full length; dn, dominant-negative; pc, pcDNA3.1; wt, wild-type.