Figure 3. Islet structure and physiological responses in transgenic MIP1-CreERT mice are preserved. Paraffin sections of pancreas were stained with anti-insulin (RED) and anti-glucagon (GREEN) antibodies using double immunofluorescence: (A) female non-transgenic; (B) female MIP1-CreERT+/−; (C) male non-transgenic; (D) male MIP1-CreERT+/−. Nuclei are stained with DAPI (BLUE); size bar in indicates 100µm for all panelsc (A-D). IPGTT performed on MIP1-CreERT+/− and non-transgenic littermate controls at 12 wk of age: (E) males and (F) females from each genotype were recorded separately. Each group consisted of 5–7 mice.