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. 2014 Jul;20(7):1014–1022. doi: 10.1261/rna.042648.113

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© 2014 Gudavicius et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — FKBP25 interacts with the pre-60S ribosome in the presence of nucleolin. (A) Sucrose density gradient ultracentrifugation of HEK293 nuclear extract. RNA and protein were extracted from each fraction and were resolved by denaturing-formaldehyde gel electrophoresis or SDS-PAGE and Western blot. (B) Sucrose density gradient ultracentrifugation of FLAG-FKBP25 immunoprecipitate from HEK293 cells. RNA and protein were extracted from each fraction and were resolved by denaturing formaldehyde gel electrophoresis or SDS-PAGE and Western blot.