FIGURE 5.

Analysis of 51G>A MOPD I-derived iPS cells. Endogenous U12-dependent introns are inefficiently spliced in iPS cells to similar extents as in MOPD I fibroblast cells. The ratio of spliced to unspliced pre-mRNA for U12- and U2-dependent introns was determined by real-time quantitative RT-PCR as described (He et al. 2011). Two MOPD I-derived iPS cell lines were compared to two MOPD I fibroblast cell lines. For each intron, the ratio seen in control fibroblasts or control iPS cells is set to 1.0. Although U12-dependent introns show increased retention in both MOPD I-iPS and MOPD I fibroblast cell lines, U2-dependent introns are unaffected in both cell lines.