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. 2014 Jul 29;9(7):e103578. doi: 10.1371/journal.pone.0103578

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© 2014 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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IGFBP-3 ELISA results for REC treated with normal glucose (NG), high glucose (HG), high glucose + TNFα (HG+TNFα), high glucose + TNFα + IGFBP-3 mutant (mutation on Serine 111/113, (HG+TNFα+BP3^Ser-Ala)), and high glucose + TNFα + IGFBP-3 siRNA + IGFBP-3 mutant (HG+TNFα+BP3si+BP3^Ser-Ala). *P<0.05 vs. NG, #P<0.05 vs. HG, ∧P<0.05 vs. HG+TNFα $P<0.05 vs. HG+TNFα+BP3^Ser-Ala. All data are mean ± SEM. Data is from 3–4 independent experiments for each treatment.