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. 2014 Jul 29;9(7):e103517. doi: 10.1371/journal.pone.0103517

Figure 6. Proteolysis of an Acm1-ProtA fusion protein in G1 requires the N-terminal 52 amino acids of Acm1.

Figure 6

A) YKA247 cells transformed with PGAL1 constructs expressing ACM1, acm1NΔ42, acm1NΔ52, acm1NΔ60, acm1NΔ72 or acm1NΔ80 fused to the ZZ domain of Protein A were arrested in G1 and protein stability assayed over time by immunoblotting with anti-Protein A antibody. B) The same assay as panel A with cells expressing Acm1 amino acids 1–52 fused to Protein A. C) The stability of Acm1 and Acm1NΔ52 without the Protein A fusion were compared using the same assay as in panel A, but with anti-Acm1 antibody for immunoblot detection. G6PD is a loading control. G6PD loading controls were performed for all blots in panels A and B as well (not shown).