Figure 4. The function of secreted miR-16 in recipient cells does not require endogenous Ago2 from the recipient cells.

A, Knockdown of Ago2 in 293T cells via Ago2 siRNA. 293T cells were incubated with MVs secreted by HeLa cells transfected with miR-16 or co-transfected with the miR-16 mimic and HA-Ago2 plasmid. B, Level of Bcl2 protein in 293T cells detected using Western blot analysis. ^*, P<0.05; ^**, P<0.01.