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. 2014 Jul 29;9(7):e103760. doi: 10.1371/journal.pone.0103760

Figure 5. CuE modulates the expression levels of multiple cell cycle, apoptosis, and signaling regulators in MDA-MB-468 and SW527 TNBC cell lines.

Figure 5

A. MDA-MB-468 and SW527 cells were treated with CuE (0–50–100–200 nM) for 24 h. Cell lysate were collected for WB, to detect PARP, cleaved Caspase-3, Survivin, Mcl-1, XIAP, Bcl-2, Bcl-xL, Cyclin B1, Cyclin D1, Cyclin E1, p21, and p27. β-actin and GAPDH were used as loading controls. B. MDA-MB-468 and SW527 cells were treated with CuE (0–50–100–200 nM) for 24 h. pSTAT3, total STAT3, pAKT, total AKT, pJNK, total JNK, p-c-Jun, total c-Jun, pERK and total ERK were examined by WB. GAPDH was used as a loading control.