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. 1978 Mar;75(3):1428–1432. doi: 10.1073/pnas.75.3.1428

Construction and properties of chimeric plasmids in Bacillus subtilis.

T J Gryczan, D Dubnau
PMCID: PMC411485  PMID: 418412

Abstract

Antibiotic resistance chimeric plasmids have been constructed by in vitro enzymatic manipulation and introduced into Bacillus subtilis by transformation. The parental plasmids used had been introduced into B. subtilis from Staphylococcus aureus by transformation. Of the seven recombinant plasmids constructed using restriction endonucleases, one was made using EcoRI, another using Hpa II, and five with Xba I (from Xanthomonas badrii), demonstrating the utility of the latter enzyme for molecular cloning experiments. Although all of the recombinant plasmids we have made replicate and express their antibiotic resistance characters, three of them have suffered a loss of DNA, either in vitro or, more likely, in vivo. The deletion event in all cases involved one of the two termini used to join the parental plasmids. The plasmid chimeras reported in this paper should prove useful for the study of plasmid replication, incompatibility, and recombination. In addition, the utility of the B. subtilis system for molecular cloning has been clearly illustrated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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