Figure 12.

In Vitro and in Vivo K_m Values versus Substrate Concentrations [S].

The substrate levels 20 min after the light shift of CBC reactions (Figure 11) are plotted against in vitro K_m (dark gray) (literature values; for details, see Supplemental Table 3) and in vivo K_m (light gray) calculated based on substrate saturation curves (Figure 11). Arrows indicate >2-fold increases between in vitro and in vivo K_m values. Enzymes with their substrate in parentheses: ^aPGK (3PGA), ^bTRK (S7P), ^cRubisco (RuBP), ^dFBA (DHAP), ^eSBA (DHAP), ^foxidized SBPase (SBP), ^greduced SBPase (SBP), ^hoxidized FBPase (FBP), ⁱreduced FBPase (FBP), ^jRPI (R5P), ^kPRK (Ru5P), ^lSBA (E4P), ^mTPI (GAP), ⁿFBA (GAP), and ^oGAPDH (BPGA). In Supplemental Figure 18, the corresponding plot with substrate levels at the low light intensity is shown.

[See online article for color version of this figure.]