Figure 14.

C Sequestration after Light Shift into Metabolite Pools and Starch.

C. reinhardtii CC-1690 cells were grown in a bioreactor at 24°C, 5% CO₂, and 41 μmol photons m⁻² s⁻¹ and shifted to 145 μmol photons m⁻² s⁻¹ at time point zero.

(A) Based on photosynthesis rate, the additionally fixed C in the higher light condition was calculated and set to 100% (n = 4 ± sd). Based on changes between metabolites pools measured by LC-MS/MS (Supplemental Data Set 1) and starch (Figure 7), the changes in C atoms in these pools were calculated and expressed relative to the additionally fixed C between all time points (see Methods).

(B) Based on the C sequestration shown in (A), the growth rate was modeled (for details, see Methods). The dashed line indicates the modeled growth rate at the low light intensity that was set to 1. AU, arbitrary units.

(C) Pearson correlation between the modeled growth rate shown in (B) and the specific growth rate measured by medium dilution rate (Figure 1A).

(D) Pearson correlation between the modeled growth rate shown in (B) and polysome loading (Figure 9).