Figure 6.

Rapid Increase of CBC Intermediates.

C. reinhardtii CC-1690 cells were grown in a bioreactor at 24°C, 5% CO₂, and 41 μmol photons m⁻² s⁻¹. At time point zero, the light was either kept at the initial light intensity (open circles, n = 4 ± sd) or shifted to 145 μmol photons m⁻² s⁻¹ (filled circles, n = 4 ± sd) at time point zero. Metabolite levels were given as concentrations in algal cells (μM; see Methods). A pairwise t test between control and treatment samples with P value correction for multiple sampling by Bonferroni correction was performed (one asterisk, P < 0.05; two asterisks, P < 0.01; three asterisks, P < 0.001). For both conditions, cells were harvested for metabolite analysis by LC-MS/MS during the 8 h following the time point zero. Metabolites levels are shown for the whole time course ([A] and [C]) and the first hour ([B] and [D]). Graphs are based on Supplemental Data Set 1.