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Increase in Polysome Loading upon Increased Light Intensity.
C. reinhardtii CC-1690 cells were grown in a bioreactor at 24°C, 5% CO2, and 41 μmol photons m−2 s−1 and kept at this light intensity (open symbols) or shifted to 145 μmol photons m−2 s−1 at time point zero (closed symbols). For each time point and both treatments, the percentage of free ribosomes (squares) and ribosomes in polysomes (cycles) was calculated based on the ribosome profile (see Methods for details; n = 3 ± sd).
