BRAT Is Robust toward Various Experimental Conditions.
Images of whole plates and selected magnifications under different experimental
conditions that lead to variations in image background color, image contrast, and
the direction of root growth. Top row (from left to right): 1× MS medium, pH 5.7, 21°C; low
temperature, 1× MS medium, pH 5.7,
10°C; high temperature, 1× MS medium, pH 5.7, 29°C; low pH, 1× MS medium, pH 4.6, 21°C; bottom row: sulfur-deficient
medium, 1× MS medium − S, pH
5.7, 21°C; iron-deficient medium, 1× MS medium − Fe, pH 5.7, 21°C;
phosphorus-deficient medium, 1× MS
medium − P, pH 5.7, 21°C; auxin transport inhibitor
(1-N-Naphtylphthalamic acid) treatment, 1× MS medium + 10 μM
1-N-naphtylphthalamic acid, pH 5.7, 21°C. White boxes in
the whole plate images indicate an area that was magnified. Bars = 1 cm.