Figure 9.

PIL1 and HFR1 Regulate the Transcription Level of PIF Direct-Target Genes in Vivo.

(A) qRT-PCR results showing the expression of PIF direct-target genes in multiple genotypes. Expression levels were normalized to an internal ACTIN2 control and are presented relative to the wild-type levels set at unity. Data are represented as the mean of biological triplicates ±sd (n = 3). All the seedlings were grown in darkness for 3 d and then transferred to red light for 20 min. 1, Wild type; 2, hfr1; 3, pil1; 4, pil1 hfr1; 5, pifq.

(B) Schematic presentation of the Dual-LUC assay reporter construct expressing LUC under the PIL1 promoter (PIL1_pro).

(C) Tobacco leaves were infiltrated with strains harboring the PIL1_pro:LUC reporter and effectors in the indicated combinations. The values were given by calculating the ratio of LUC activities to REN activities (LUC/REN). Error bars represent ±sd (n = 3).