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TUNEL Assay Showing Defected Tapetal Program Cell Death in the ms142 Mutant.
DNA fragmentation signals (yellow fluorescence) started at the tetrad stage and exhibited obvious positive signals at the young microspore stage in TNG67. No DNA fragmentation signal was observed in the tapetum of ms142. The red signal is propidium iodide staining, and the yellow fluorescence is the merged signal from TUNEL (green) and propidium iodide (red). Bars = 50 μm.
