Figure 2.

Reduced Pollen Growth and Seed Content in STIG1 RNAi Plants.

(A) Wild-type pistils or transgenic pistils were hand-pollinated with wild-type pollen, dissected at 6 h, and stained with decolorized aniline blue to visualize pollen tubes. Yellow dashed lines indicate the growth front of pollen tubes. Bars = 1 mm.

(B) Quantitative RT-PCR of STIG1 mRNA levels, using total RNA of mature stigmas. n = 3 independent experiments.

(C) In vivo pollen tube lengths in (A). n = 3 independent experiments. At least six pistils were observed for each experiment.

(D) Seed content per fruit in self-pollinated STIG1 RNAi plants. n = 3 independent experiments. At least 10 fruits were harvested for each line in each experiment.

For (B) to (D), asterisks indicate significant differences from the wild type (P < 0.05, Student’s t test). Error bars indicate se.