Figure 3.

Seed Erucic Acid Content, FAE1 Expression Level, and DNA Methylation Status of FAE1 Alleles of Lines Y496 (E²E²), Y1130 (E³E³), 5-azaC–Induced Revertants (E³E³), and the Spontaneous Revertant (E³E³) of Y1130 in Yellow Mustard.

(A) Erucic acid contents of Y496, Y1130, 5-azaC–induced revertants, and spontaneous revertant of Y1130.

(B) FAE1 expression in the 21-d-old embryos of Y496, Y1130, 5-azaC–induced revertants, and spontaneous revertants of Y1130.

(C) DNA methylation was not detected in the promoter region (nucleotides −335 to 1) of E¹ in Y517.

(D) DNA methylation was not detected in the promoter region (nucleotides −5234 to −4900) of E² in Y496.

(E) DNA methylation was detected at positions −5146, −5095, and −4943 in the promoter region (nucleotides −5234 to −4900) of E³ in Y1130.

(F) and (G) DNA methylation was detected at position −4943 in the promoter region (nucleotides −5234 to −4900) of E³ in the 5-azaC induced and spontaneous revertants of Y1130.