Skip to main content
. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Cell Microbiol. 2014 Apr 15;16(8):1267–1283. doi: 10.1111/cmi.12284

Figure 7.

Figure 7

FmqD is a non-covalvent cell wall-associated protein. A) Western blot analysis of non-covalent cell wall extracts from WT (lanes 1 and 5), OEH2A::GFP (lanes 2 and 6), and OEFmqD::GFP (lanes 3 and 7) isolated from 48 and 72 hours grown cultures using primary monoclonal GFP antibodies. Lanes 4 and 8 depict 10 ng of purified C-terminal epitope-tagged GFP. B) Western blot analysis of culture filtrate extracts from WT (lanes 1 and 4), OEH2A::GFP (lanes 2 and 5), and OEFmqD::GFP (lanes 3 and 6) isolated from 48 and 72 hours grown cultures using primary monoclonal GFP antibodies. Fusion protein sizes on blot: H2A::GFP (45 kDa), FmqD::GFP (82 kDa).