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. Author manuscript; available in PMC: 2015 Jul 17.
Published in final edited form as: Br J Dermatol. 2014 Jul 17;171(1):30–38. doi: 10.1111/bjd.12899

Fig. 3. 12/15-LOX deficiency reduces densities of Sca1+CD106+ MSCs in the dermis of wounded skin.

Fig. 3

Images in (a) and (b), images in (c) and (d), and image (e) and (f), are for 3, 8 and 14 dpw, respectively. Images (g) and (h) are for sham. Images in (a), (c), (e) and (g), and images in (b), (d), (f) and (h), are for C57BL/6 (WT) and 12/15-LOX−/− mice, respectively. Immunofluorescent co-staining and microscope imaging were conducted on cryosections of each wound with 10 mm margin of surrounding skin, or skin at the equivalent location of sham. (i) There are more cells co-stained (yellow) of Sca1 and CD106 (i.e, Sca1+CD106+ MSCs) in the wounded dermis of WT mice compared to 12/15-LOX−/− mice for 3, 8 and 14 days post-wounding. Each value is the average number per HPF of Sca1+CD106+ MSCs from six mice per group ± SD. *p < 0.05 and **p < 0.01 are for WT versus 12/15-LOX−/− mice; #p < 0.05 and ##p < 0.01 are for the wounded versus sham of the same type of mice. HPF, high power field (63X); d, dermis; hf, hair follicle; Big yellow arrow, incisional wound and direction from epidermis to hypodermis of skin (a–f); Scale bar = 28 μm.