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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Mol Cell Cardiol. 2014 May 15;74:103–111. doi: 10.1016/j.yjmcc.2014.05.004

Figure 5.

Figure 5

Loss of RCAN1 sensitizes cardiac myocytes to I/R damage. Calcineurin inhibition restores protection. Depletion of RCAN1 proteins from NRVMs was verified by western blot 48 hours after transfection with control (si-control) or RCAN1-targeted si-RNA (si-RCAN1) (A). Tubulin was used as a loading control. Schematic depicts the parameters for simulated I/R (B). LDH release into the media was used to quantify cell death following sim-I/R. Cultures were treated with vehicle or FK506 prior to sim-I/R as indicated (C). A cardiomyocyte-specific RCAN1 transgene (TG) conferred protection from I/R damage in PM operated animals, but provides no increase in protection to AM-operated animals (D) (n=5 each genotype and time point). Injection of FK506 just prior to reperfusion confers protection to the PM-operated animals (E) (n=5 each treatment).